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microscopic cover chambers  (Labtek)

 
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    Labtek microscopic cover chambers
    Microscopic Cover Chambers, supplied by Labtek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microscopic+cover+chambers/microscopic+cover+chambers/pm24522395-44-5-7
    Average 90 stars, based on 1 article reviews
    microscopic cover chambers - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: A simple method to estimate the average localization precision of a single-molecule localization microscopy experiment.
    Article Snippet: single-molecule fluorescence (Orrit and Bernard 1990), today, the huge field of single-molecule biophysics emerged.. This includes single-molecule localization microscopy (SMlM) techniques (Betzig et al. 2006; rust et al. 2006; Heilemann et al. 2008), in which the total fluorescence signal is detected as subsets of single fluorophores employing photoswitching. an important parameter for SMlM experiments is how accurate the positions of the detected fluorescent emitters can be determined, which directly influences the achievable resolution.. For the quantification of the localization precision, several strategies were proposed.



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    TPEN increases intracellular zinc levels. A. parasiticus wild-type SU-1 spores were center inoculated onto solid GMS media with and without 200 μM TPEN and were grown at 30 °C for 5 days. Plugs containing fungal colony and media were taken from GMS with and without TPEN and incubated with 10 μL Zinpyr-1 (5 μM) probe for 30 min at 37 °C and examined under a fluorescent <t>microscope.</t> The stained cells were observed under the fluorescent microscope as described in Methods. The accumulation of green fluorescent Zinpyr-1 probe was detected within the intracellular matrix of fungal mycelia. Bar 10 μm.
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    TPEN increases intracellular zinc levels. A. parasiticus wild-type SU-1 spores were center inoculated onto solid GMS media with and without 200 μM TPEN and were grown at 30 °C for 5 days. Plugs containing fungal colony and media were taken from GMS with and without TPEN and incubated with 10 μL Zinpyr-1 (5 μM) probe for 30 min at 37 °C and examined under a fluorescent microscope. The stained cells were observed under the fluorescent microscope as described in Methods. The accumulation of green fluorescent Zinpyr-1 probe was detected within the intracellular matrix of fungal mycelia. Bar 10 μm.

    Journal: Toxins

    Article Title: Effects of Zinc Chelators on Aflatoxin Production in Aspergillus parasiticus

    doi: 10.3390/toxins8060171

    Figure Lengend Snippet: TPEN increases intracellular zinc levels. A. parasiticus wild-type SU-1 spores were center inoculated onto solid GMS media with and without 200 μM TPEN and were grown at 30 °C for 5 days. Plugs containing fungal colony and media were taken from GMS with and without TPEN and incubated with 10 μL Zinpyr-1 (5 μM) probe for 30 min at 37 °C and examined under a fluorescent microscope. The stained cells were observed under the fluorescent microscope as described in Methods. The accumulation of green fluorescent Zinpyr-1 probe was detected within the intracellular matrix of fungal mycelia. Bar 10 μm.

    Article Snippet: Under minimal light exposure, the plugs were placed into a two-chambered cover glass microscope slide (Thermo Scientific Lab-Tek II, Waltham, MA, USA) and 10 μL of the probe or DMSO control was pipetted immediately on to the plug.

    Techniques: Incubation, Microscopy, Staining