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microscopic cover chambers  (Labtek)

 
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    Labtek microscopic cover chambers
    Microscopic Cover Chambers, supplied by Labtek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microscopic cover chambers/product/Labtek
    Average 90 stars, based on 1 article reviews
    microscopic cover chambers - by Bioz Stars, 2026-04
    90/100 stars

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    microscopic cover chambers  (Labtek)
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    Labtek microscopic cover chambers
    TPEN increases intracellular zinc levels. A. parasiticus wild-type SU-1 spores were center inoculated onto solid GMS media with and without 200 μM TPEN and were grown at 30 °C for 5 days. Plugs containing fungal colony and media were taken from GMS with and without TPEN and incubated with 10 μL Zinpyr-1 (5 μM) probe for 30 min at 37 °C and examined under a fluorescent <t>microscope.</t> The stained cells were observed under the fluorescent microscope as described in Methods. The accumulation of green fluorescent Zinpyr-1 probe was detected within the intracellular matrix of fungal mycelia. Bar 10 μm.
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    TPEN increases intracellular zinc levels. A. parasiticus wild-type SU-1 spores were center inoculated onto solid GMS media with and without 200 μM TPEN and were grown at 30 °C for 5 days. Plugs containing fungal colony and media were taken from GMS with and without TPEN and incubated with 10 μL Zinpyr-1 (5 μM) probe for 30 min at 37 °C and examined under a fluorescent microscope. The stained cells were observed under the fluorescent microscope as described in Methods. The accumulation of green fluorescent Zinpyr-1 probe was detected within the intracellular matrix of fungal mycelia. Bar 10 μm.

    Journal: Toxins

    Article Title: Effects of Zinc Chelators on Aflatoxin Production in Aspergillus parasiticus

    doi: 10.3390/toxins8060171

    Figure Lengend Snippet: TPEN increases intracellular zinc levels. A. parasiticus wild-type SU-1 spores were center inoculated onto solid GMS media with and without 200 μM TPEN and were grown at 30 °C for 5 days. Plugs containing fungal colony and media were taken from GMS with and without TPEN and incubated with 10 μL Zinpyr-1 (5 μM) probe for 30 min at 37 °C and examined under a fluorescent microscope. The stained cells were observed under the fluorescent microscope as described in Methods. The accumulation of green fluorescent Zinpyr-1 probe was detected within the intracellular matrix of fungal mycelia. Bar 10 μm.

    Article Snippet: Under minimal light exposure, the plugs were placed into a two-chambered cover glass microscope slide (Thermo Scientific Lab-Tek II, Waltham, MA, USA) and 10 μL of the probe or DMSO control was pipetted immediately on to the plug.

    Techniques: Incubation, Microscopy, Staining